This diissecting microscope is used to prepare samples for electron microscopy, particularly for high pressure freezing applications. This instrument would have to be used independently by the HDDC member. Although we have this instrumentation, there is currently no expertise in the core for cryo ultramicroscopy. The microprocessor controller can handle up to 15 programs with up to 25 steps each.Ĥ) Leica IP S Automated Slide Printer, which is an ink-jet printing module that is used for high-throughput printing of labels for histology slides.ĥ) Leica CM 1850 Cryostat, which is used to prepare sections from frozen tissue samples for immunostaining and analysis by confocal microscopy or for immunoperoxidase methods.Ħ) Leica RT2125 Rotary Microtomes (3), which are used to cut paraffin sections.ġ) Ultracut E Ultramicrotomes (2), which are used to cut thick and ultrathin plastic sections.Ģ) Ultracut-S Ultramicrotome, which is used to cut thick and ultrathin sections from frozen tissue. The instrument has self-contained fume control with a replaceable filter. The machine also allows for simultaneous staining of several different protocols - the user can select any compatible program for each rack. The capacity is 150 slides per hour with a typical H&E protocol. The instrument has the capability of continuous loading - it can load up to 11 racks (with 30 slides per rack) while processing. There are18 reagent stations, 5 wash stations, and an integrated forced hot air oven that significantly reduces slide drying time. It has the capability of programming numerous special stains or customer-specific modifications of the H&E stain. This equipment is used to stain paraffin sections. It additionally has a compressor-cooled cold spot for cooling blocks and a melted paraffin holding reservoir for the newly processed cassettes. The machine has a separately heated paraffin dispensing system. This equipment is used to embed tissues/cells that are infiltrated with paraffin. The machine also has the capacity to interrupt an automatic process for reloading or removing cassettes for special applications before the end of a run.Ģ) Leica EG1160 Embedding Center Dispenser and Hot Plate There is a delayed start function up to 9 days this allows processing over the weekend to be ready on Monday morning. Infiltration time is separately programmable for each station.
Two loading baskets can be run each night with an 80 cassette capacity per basket. The carousel configuration has 12 stations 10 for reagents and 2 for melted paraffin.
The instrument has vacuum function and a fume control system. This tissue processor is used to dehydrate and infiltrate tissues/cells with paraffin. Maps and other information about access, pricing, and services can be found at. Dana Research Building on the East Campus of the BIDMC (please note: this is NOT at the Dana Farber Cancer Center!!) and consists of more than 2,000 square feet of space. The HDDC Core B at BIDMC is located on the 8th floor of the Charles A. Substantially discounted rates for HDDC users will return as a membership benefit on April 1, 2022. The fee schedule for HDDC members (July 1, 2021-March 31, 2022) include priority access and service for all HDDC users with no overhead charges for users with labs outside of the BIDMC. In addition, the microscopy core has an immunostaining service.Īll work is done on a fee-for-service basis to recover costs for equipment upkeep, supplies, and technical assistance. The Histology core, provides service for paraffin embedding, sectioning, staining, and frozen sectioning and the Imaging/Microscopy core, provides shared equipment and service for confocal, widefield, photodocumentation, electron microscopy, and digital image processing. The HDDC Imaging Core B at the Beth Israel Deaconess Medical Center (BIDMC) consists of two interacting components:
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